Background: Malnutrition is due to an unbalanced or inadequate diet. Mostly Preschoolers are more prone to affected by malnutrition. 7 out of 10 children in the community are malnourished. The lack of proper education and training in the field of nutrition among health care professionals has been a longstanding issue that has been highlighted in various publications. Health workers play an important role in promoting growth and development of children and to prevent malnutrition. Objectives: To find out the effect of computer-assisted teaching among the health workers of selected community health centers regarding the prevention of malnutrition. Methods: pre-experimental (one group pre-test post-test) design was used. Convenient type of sampling technique was used to select fifty no. of health workers of selected CHC of Bhubaneswar, Odisha as study sample and Self-structure questionnaire was used to collection of information. Result: Analysis is revealed that computer-assisted teaching improved the knowledge of health workers regarding the prevention of malnutrition (before intervention 39.93±11.86 & after intervention 77.66±7.76) significantly as calculated “t” value is 32.19 & calculated p-value was ˂0.0001 which is less than 0.05 level of significance. A statistically significant association was there in between the knowledge level with sociodemographic variables such as educational qualification & exposure to nutritional program. Conclusion: On the finding of the study, it was found that computer-assisted teaching is more practical in upgrading the knowledge of health workers on prevention of malnutrition which ultimately can improve the health status of children and prevent malnutrition.