Objectives: 1. To assesses the anxiety level in both the experimental and control group before and after play session.2. To compare the level of anxiety among hospitalized preschool children between the experimental and control group. 3. To find out the association of anxiety children with selected demographic variables. Methods: Quasi-experimental non-randomized control group research design was adopted for this study. A total of 50 pre-school children were selected by using purposive sampling technique in IMS & Sum hospital Bhubaneswar. The level of anxiety was assessed using a self-structured questionnaire. Results: This study showed in the experimental group, before play session about 20%, 64%, 16% pre-school children had mild, moderate anxiety, severe anxiety respectively & after a play session, on post-test results showed 76% preschool children had mild anxiety, 16% preschool children had moderate anxiety & no one had severe anxiety whereas in control group pre-test results showed 60%,28%,12% preschool children had mild, moderate, severe anxiety respectively & on post-test results showed 48%,36% &12% preschool children had mild anxiety, moderate & severe anxiety respectively. Before the play session, in the experimental & 7control group, the mean ±SD value of anxiety score was 29.6± 6.55 and 24.52±8.06 respectively. The obtained “t” =2.44 with p-value is 0.0091 which was statistically significant, whereas in post-intervention the mean ±SD value of anxiety score in experimental was 19.8±4.64 and in control group mean ±SD value was 25.04±8.12. The obtained “t” =2.80 with p-value was 0.0073 which was statistically significant. Conclusion: Organized Play session is an effectual session in reducing anxiety among hospitalized preschool children.