Background: Infertility is one of the most significant problems in gynaecology. The present study was evaluated FSH, LH and prolactin hormones in female infertility. Materials & Methods: 72 females with infertility were enrolled in group I and age matched healthy controls were included in group II. The levels of serum follicle stimulating hormone (FSH), luteinizing hormone (LH), and prolactin were measured with enzyme-linked immune-sorbet assay (ELISA) methods.