Nanotechnology has become an emerging tool of sophisticated strategy in the current scientific era and has become a major contributing factor to the field of therapeutics. Wound infections are one of the most common infections with the ability to cause significant mortality and morbidity. The increase in the emergence of drug resistance has made the researchers to look for an alternative strategy and in this scenario; nanotechnology combined with the traditional medical plants can serve as the best candidate for an alternative regimen to the current chemotherapy. In this study, Pus samples were collected from Hospitals and the pathogens were isolated and identified. Then Silver Nanoparticles were synthesized from Tinospora cordifolia and their antibacterial efficacy against wound pathogens was tested after Characterization by employing UV-VIS Spectroscopy, XRay Diffraction, FTIR, TEM and EDX. The reports suggested that Silver nanoparticles exhibited potential antibacterial activity against the pathogens like Pseudomonas aeruginosa and Staphylococcus aureus. In the future, these synthesized nanoparticles of Tinospora cordifolia candidates for treatment against pus pathogens