Now a day’s health care cost has been increasing very rapidly. The healthcare services, hospitals and clinics cost for providing services to their patients have been increased very sharply. Therefore, these organizations are always struggling to cut down the cost so that high quality services can be provided to every segment of the people in less price. By doing this, every patient can get good services from the hospitals or clinics which will be monetarily under their reach. 5S methodology helps to achieve the goal of reduction in time, wastages and non-operative time. Which results in cost savings and good services to their patients. This enhances the productivity of the nursing staff and makes organization effective and efficient. A sample of total 40 nursing staff were selected among the existing hospitals in Nagpur city. The nursing staff comprising males and females were considered for the study. To find out the impact of 5S before and after implementation on the productivity of the nursing staff in hospitals, paired sample t-test was used.The research study has shown the influence of the 5S in enhancing the productivity of the nursing staff in terms of reduction in staff non-operative time, staff overtime, patients waiting time, theatre preparation time and cost involved in the processes.