Human Immunodeficiency Virus type-1 (HIV-1) is that the explanation for Acquired Immune Deficiency Syndrome (AIDS), a serious human viral disease.The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population within the world. The aim of the present study was to evaluate the in vitro anti-HIV activity of selected Indian medicinal plant extracts.Extracts were prepared from dried plant Vitex negundo, Evolvulusalsinoides and Datura metel leaf with Hexane, Methanol, Ethanol, Chloroform, and Petroleum ether. The phytochemical activities and HIV-1 RT inhibition activity (HIV-1 RT colorimetric ELISA kit –Roche Kit method) was carried with the all solvent extracts of Vitex negundo, Evolvulusalsinoides and Datura metelwas determined. The methanolic extracts of Vitex negundo have given the maximum and positive result with 74.76±1.36, which proves that Vitex negundo has a potential antiviral activity against HIV. Phytochemical analysis conducted on the plant extracts revealed the presence of constituents which are known to exhibit medicinal also as physiological activities.The predominant components present altogether the extracts of Vitex negundo are flavonoids, Terpenoids and steroids. Thus this study seems to justify the normal use of plant for the treatment of communicable disease of viral origin.