Abstract: Present study is to assess the incidence of hypomagnesemia and hypocalcemia on day one and day four in neonates who are born in a tertiary care hospital of western maharashtra. Study done at KRISHNA Hospital, Karad. Children s who were born congenitally, and who met the eligibility criteria, were studied in the obstetric department of Krishna Hospital. 2.5 ml of cord blood was collected on day 1 of life in a plain bulb. On Day 1 of life, Hypocalcemia occurred in 0.5% of the 400 neonates studied. On Day 1 of life, Hypomagnesemia occurred in 1% of the 400 neonates studied. On Day 4 of life, Hypocalcemia occurred in 10.5% of the 400 neonates studied. On Day 4 of life, Hypomagnesemia occurred in 0% of the 400 neonates studied. No cases of Hypocalcemia with Hypomagnesemia occurred together at the same time either on Day 1 of life or on Day 4 of life.