The marine microbes isolated from sedimented specimens from various places of Pichavaram, Parankipettai, and Muthupet in India. Antibacterial substances from marine bacteria were identified by cross streak method and agar plate technique. The potent marine bacteria species were inoculate on production medium and filtered. The extract was screened for antibacterial activity. Nine marine bacteria species were isolated from the selected marine sediment sample. All the nine isolates were active against the test organisms. Chemical screening strongly suggested that presence of alkaloids, Carbohydrates and Glycosides. The result of marine bacteria is composed of potent secondary metabolites. Isolation, screening of marine bacteria can be useful in discovery of novel metabolites.