Abstract. Postprandial hyperglycemia is a significant risk factor involved in type 2 diabetes. The elevated level of postprandial hyperglycemia is caused by carbohydratehydrolyzing enzymes, α-amylase, and α-glucosidase. Inhibition of these enzymes appears to be an effective means for diabetes management. Recent studies suggested that endophytic fungi of some medicinal plants produce α-glucosidase and α-amylase inhibitors. The endophytic fungus P. brevicaule alba-CC 200, which exposes inhibition of α-amylase, was isolated from the Celosia cristata plant. This article presents data on the purification of inhibitory compounds from the methanolic extract of Penicillium brevicaule alba ThomCC200. Fraction of G-7 with the highest inhibitory activity of 76.5% isolated by adsorption column chromatography. The yield of dry samples was more than 18 weight % (w/w) with more than 60% purity. The HPLC and LC-MS of fraction G-7 indicated that the inhibitory activity is these fractions associated with the triterpene saponins.