Probiotics are refers to as microorganisms which have many health benefits.The most probiotics are lactic acid bacteria and some other groups. These probiotics are present in higher amount in milk and milk products. In this study the numerous groups of probiotic bacteria have been identified and isolated in food and food products especially in dairy products such as milk, cheese, kefir, acidophilus milk, yogurt etc… Probiotics are gram positive rods or cocci aerotolerant anaerobes / facultative anaerobes, non – motile, non -sporing and non motile. They have high tolerance to stomach pH than other microbes. These strains were isolated using De Mann Rogosa Sharpe (MRS) agar medium and incubated at 37°C for 4i hours separately under aerobic and anaerobic condition. After growth they were isolated, purified and characterization were done based on morphology analysis such as gram staining and biochemical analysis. The probiotics are useful for both humans and animals. They have several health benefits such as improve immune response, many antibiotic activity, antioxidant activity etc. Probiotics contains more B vitamins and also helps in lactose metabolism and food digestion.