The non-experimental research study was aimed to assess the knowledge & practice of staff nurses on prevention of UTI among patients with an indwelling catheter in Bhubaneswar with objectives to assess the level of knowledge of staff nurses on prevention of UTI among patients with an indwelling catheter, to assess the level of practice of staff nurses on prevention of UTI among patient with an indwelling catheter, to find the association between knowledge and practice of staff nurses with a selected socio-demographic variable. “Cronbach’s Alpha” formula was used to calculate the reliability. The value for knowledge was 0.84 & practice regarding prevention of UTI was 0.8. Which indicates the tool was reliable and acceptable. The study was based on a questionnaire method with 250 samples selected by random sampling technique. A self-structured questionnaire was used for knowledge with score poor (1-21), Average (22-42), Good (43-64), Very good (65-84). Practice with a score very poor (0-5), poor (5-10), good (11-15). Initially, the level of knowledge and practice of staff nurses were assessed by an administered questionnaire. In the analysis, there was statistically remarkable to the association between practices of staff nurses with the selected socio-demographic variable at p-value 0.0005. This study conducted that there was a strongly significant association between the ages of the staff nurses with practice regarding the prevention of CAUTI.