Background: According to the statical data maximum number of the surgical operated patients are cholecystectomy patients with gall stone having the major cause of the disease. Acute and chronic inflammation of the gall bladder is the most common complication of the gallbladder stone disease. A bacterial cause has been proposed and bacteria are cultured in most of the patients with acute cholecystitis. While the reported incidence of positive bile culture in patient with chronic cholecystitis who had undergone elective operation on the other hand is less, whereas evidence also suggests that normal bile is sterile. So identifying the microflora will help to identify the probable cause and to choose appropriate antibiotics. Methods: A prospective study was done on 80 patients for a period of one year of surgery in association with microbiology department, all the patients undergoing laproroscopic cholecystectomy acute and chronic were taken for taken, bile gall bladder wall and the stones were sent to the laboratory for culture. Results: In the present study 80 patients were evaluated. Out of which maximum number of patients were clustered between 35-45 years (25 patients), 70(%) were females and 10 (%) were males. We found 31.25% bile culture positive, 51.25% gall stones were positive and gall bladder wall was positive for culture in 57.5% The commonest organism was Staph Aureus(35%) then E. coli (10%) followed by streptococcus (7.5%) Conclusions: Person with the biliary tract disease harbor bacteria in the gall bladder stone , bile and the wall of the gall bladder , so it’s important in choosing the antibiotic to prevent complication in only minimum number of the patients and as the stones also harbor the microorganism so the stones should not be handed over the attendants’ and the patients.