Introduction: Anencephaly occurs due to the complete absence of cranial vault and subsequent disruption of the cerebral cortex with a severely damaged brain. In anencephaly, the forebrain and brain stem are exposed. Forebrain either does not develop or is destroyed, leading to the absence of cerebrum and cerebellum. Material and method: NTD were taken in the study group. During the autopsy clinical findings, external examination, internal examination and photography were done along with the histopathology of the specimens. Total RNA from NTD patient and control was extracted using acid guanidinium thiocyanate-phenol-chloroform extraction Result: Demonstrated here the differential expression of specific genes like WNT11, ROR-2, PAX3/PAX7, SOX10, RET associated with development of the neural tube affected and normal adjacent tissues.