Background: Childbirth is in essence a joyous moment, it puts the women to experience the extreme form of discomfort. The mother undergoes a wide range of pain in labor and expresses the correspondingly different extent of reactions to it. A woman’s response to labor pain may be determinant of the conditions of her labor process, the setup of the birthing unit, her ethnicity, race, lifestyle and pre-pregnancy construct concerning a perception of pain & anxiety in primi and multi mothers and to compare the level of perception of pain & anxiety among both. Objectives: We studied the level of perception of pain & anxiety in primi & multi mothers and compared the level of perception of pain & anxiety in primi & multi mothers to see the association between level of perception of pain & anxiety with other variables. Methods: A comparative descriptive survey design was adopted and by non-probability convenient sampling technique 80 primiparas and 80 multiparas were sampled. A 3 point Wong-Baker rating face scale for pain assessment and modified 3 point Hamilton anxiety rating scale for anxiety level assessment was used. Result: In both the groups 52.50% of primipara had severe pain and 10% of multiparas had severe pain in the first stage of labour. 70% of primipara had severe anxiety and 12.50% of multipara had severe anxiety in the first stage of labour. The difference in the degree of pain perception in primi and multi was significant at ‘p’ value 0.000316 & anxiety is <0.0001. None of the socio-demographic variables appeared to be related to the level of perception of pain & level of anxiety.