Background:The idea of doing single-visit root canal treatment is not new, however most accepted approaches in current use emphasize on multi-visit systems. Aim: To evaluate the perception of Single-visit Root Canal Treatment among the dental practitioners in southern Indian region. Methodology: This was a descriptive cross-sectional survey which include 210 dental practitioners regardless of their specialty in southern Indian region. Data collection was done by sending structured self-administered questionnaire to the dental practitioners by different means of social media (WhatsApp, Email, Facebook etc.). All data was recorded and evaluated by using SPSS software. Results: A total of 210 dental practitioners responded to the survey, the response rate was 84%. Majority 101 (48.1%) of the dental practitioners responded vitality of pulp was the reason for choosing the single-visit RCT. When the dental practitioners were asked about the reason for choosing multiple-visit RCT, most of them responded that the tooth with periapical lesion/Retreatment 63 (30%) was the reason. Conclusion: Most dental practitioners of southern Indian region perform single-visit root canal treatment in cases of vital pulp, but most of the dental practitioners perform multiplevisit root canal treatment in cases of necrotic pulp.