Background: Antimicrobials resistance occurs when a drug loses its ability to inhibit bacterial growth effectively and poor knowledge of guardian towards it is one of the leading causes of antimicrobials resistance in their children. Purpose of the study: The present study aims to improve knowledge, attitude and practice towards antibiotics use among guardians of pediatric patients attending in tertiary care hospital, Pokhara, Nepal. Methods: An interventional study among 86 guardians was conducted in pediatric ward of hospital using census technique. KAP of guardians before and after the educational intervention was studied to compare KAP score. KAP scoring was done on the basis of Likert scale. Additional information for the study was obtained from the patient medical file. Results: The average age of the guardians was 28.59 years (SD±5.785) and majority (83%) belonged to Hindu religion. Result showed that (82.6%), (91.9%), (91.9%), (73.3%) and (10.5 %) parents had knowledge about commonly used medications Antibiotics, Analgesics-antipyretics, analgesics, antihistamine and normal saline nasal drops, respectively. The median knowledge, attitude and practice scores after the intervention were 16, 23 and 26 respectively while the overall median score increased to 65. By using the Wilcoxon signed ranks test, knowledge (p<0.001), attitude (p<0.001), practice (p<0.001) and total scores (p<0.001) increased after the intervention. Conclusion: Despite of good knowledge and positive attitude regarding antibiotics use and resistance but good practice is very low. Therefore there is need to educate parents on the rational use of antibiotics with further interventional study.