Abstract Heat shock proteins (HSPs) are a large class of proteins that have been conserved throughout evolution, present in all organisms studied so far, and are a major immunity in infections caused by diseases including leishmaniasis. This study aimed to produce and develop the rhsp70 recombinant from Leishmania major parasite using advance gene synthesis technique and traditional recombinant methods. These proteins were then determined with the Western blot technique and testing the immune stimulation experimentally. Groups of mice were injected with (rHSP70), and control the results were compared between those groups and between them with the positive and negative control groups, the results showed significant a difference between those groups, as the groups that were injected before infection with a concentration of 25 µg/ ml and 50 µg/ ml showed significant changes with all other groups The groups that were injected with a concentration of 75 µg/ ml and 100 µg/ ml showed significant changes with all groups except for the groups that were injected with a concentration of 75 µg/ ml and 100 µg/ ml after infection.