Health in its broadest sense is a dynamic state in which the individual adapts to change in internal and external environments to maintain a state of well-being. Night shift work is essential to the operation of a hospital. Several studies have shown that working on a night shift as a permanent shift or rotating shift affects the health and well-being of the workers. Night shift workers report poorer health than their day shift counterparts. A descriptive comparative study was conducted in IMS &SUM Hospital, Bhubaneswar. 86 staff nurses randomly selected for this study. The objectives of the study were to assess health and wellbeing of night shift nurses working on different intensive care units. To assess health and well-being of night shift nurses working on emergency. To find out the difference between health and wellbeing of night shift nurses working on different medical and surgical ICU. To find out the difference between health and well-being of night shift nurses working in ICU and emergency. The study population were emergency staff nurses, medical and surgical ICU staff nurses of Sum nursing College, Bhubaneswar. Selfstructure socio-demographic Performa, knowledge and experience questionnaire was used to assess the association of knowledge and experience with socio-demographic variables. The data was analyzed by using SPSS version 11.5. The result of the studies revealed that 43% having very hard night shift duty,23.36% having hard night shift duty,8.6% having moderate night shift duty. The present study revealed that age, marital status, total exposure, nurse-patient ratio having no significant relationship with health. In this study revealed that age, educational status, experience, nurse-patient ratio having no significant relationship with well-being