Background: The present study aims to assess correlation of sleep patterns, melatonin secretion, and obesity. Materials and Methods: One hundred and twenty-two subjects in age ranged 11-17 years of both genders were subjected to fasting blood glucose, insulin level, uric acid, lipid profile and renal function test measurement. Insulin resistance and level of melatonin with enzyme-linked immunosorbent assay (ELISA) were determined. Results: There were 30 males and 22 females without insulin resistance (IR) and 40 males and 30 females with IR. There were 6 prepubertal, 12 pubertal, and 34 postpubertal subjects without IR and 8 prepubertal, 15 pubertal, and 47 postpubertal subjects with IR. The mean Body Mass Index (BMI) (Kg/m2) was 29.1 and 34.2, Systolic Blood Pressure (SBP) (mm Hg) was 128.6 and 134.2, Diastolic Blood Pressure (DBP) (mm Hg) was 66 and 72, total cholesterol (TC) (mg/dl) was 160.2 and 164.8, triglyceride (TG) (mg/dl) was 70 and 104.2, High Density Lipoprotein (HDL) (mg/dl) cholesterol was 54.2 and 46.8 and Low-density lipoprotein (LDL) (mg/dl) cholesterol was 92 and 98 in subjects without IR and in subjects with IR respectively. The mean uric acid was 5.3 mg/dl in subjects without IR and 5.9 mg/dl in subjects with IR. The mean MT6s:Cr (Melatonin 6s: Creatinine) ratio was 31.4 in subjects without IR and 27.4 in subjects with IR. The difference between both groups was found to be significant (P< 0.05).