Pyridine is one of the natural compounds that it builds upon in the formation of other chemical compounds in a number of applied industrial fields such as agricultural chemistry and the pharmaceutical industry. Pyridine is used in industry as a chemical solvent and reagent; And also in DNA synthesis in the laboratory. Historically, pyridine was obtained from coal tar and as a by-product of the coal gasification process; However, the increased demand for it has led to the development of synthetic reactions of simple compounds such as acetaldehyde and ammonia. Annually, more than 20 thousand tons of the compound are produced worldwide. Series of Pyridine –heterocyclic derivatives were prepared via cyclization reaction and condensation reaction, then identification all prepared new compounds via several techniques (FT.IR, H.NMR, Mass)– spectrophotometric, other physical and chemical properties, with microbial studying for all new prepared pyridyl derivatives.