Introduction: Breast milk is the vital nutrient for the new born baby all that they required. Some mothers are not able to feed their baby or some babies are not have that privilege to have their own mother’s milk, that time another mother’s milk is also a boon for that boon become reality Breast milk Donation is necessary. Human Breast Milk Donation is the bridge between helpful to needed one(Mother’s milk is considered very essential for the newborn infant explain with reason., n.d.). It provides total nutrient requirement for the first six months of life. It also prevents malnutrition and allows the child to develop fully. But when there is not enough mothers’ milk available due to some reasons like mother has insufficient milk glands, has had past breast surgery or is taking medication (e.g. chemotherapy for cancer) and has an infection that could spread to her baby through breast feeding. For these babies an alternative must be sought.(Ghuge et al., 2018) The present study title: A study to assess the knowledge and attitude regarding human milk donation among postnatal mother in selected hospitals of Pune city. The objective of study to assess the knowledge and attitude regarding Human breast milk donation, association between variables and associate those findings with demographic variables. Material and Methods: In present study, researcher adopted Non-Experimental descriptive design using structured questionnaire and Likert scale. Non probability convenient sampling technique used for data collection. Data analysis was done mainly using descriptive statistics. Result : The finding of the study reveals that majority of 46% postnatal mothers were from under the age group of 23-26 years, mothers do not having previous knowledge about Human breast milk donation. Present study shows that Mean knowledge score of the postnatal mothers regarding Human milk donation shows 8.89 with 3.06 Standard deviation. Result shows that mean attitude score of the post- natal mothers regarding Human milk donation shows 28.56 with 9.62 Standard deviation. co- relation between knowledge and attitude regarding Human Milk Donation was negative co There is no significant association between knowledge regarding Human Milk and Donation demographic variable Age (Years), Education, Type of Family, Occupation, Family Income per month and Area of Residence at 0.05 level of significance. Therefor the Ho2 has accepted. there is no significant association between attitude score of mothers regarding Human Milk and Donation demographic variable Age (Years), Education, Type of Family, Occupation, Family Income per month and Area of Residence at 0.05 level of significance. Therefor the ho2 hypothesis has accepted. Conclusion: The knowledge of postnatal mothers regarding Breast milk donation was average nearly poor level. The attitude of postnatal mother is almost divided in to highly favorable , moderately favorable, unfavorable. Knowledge and Attitude has no co relation in this study.