Introduction: Food safety is an important public health issue to prevent or control food- borne illnesses. In response to the increasing number of food-borne illnesses, governments all over the world are intensifying their efforts to improve food safety. According to the WHO, 1.5 billion cases of children suffer from diarrhea each year and 3 million premature deaths. However, these deaths and illnesses are shared by both developed and developing nations. The Centre for Disease Control and Prevention (CDC) estimated that food- borne diseases caused approximately 76 million illnesses annually among the United States of America’s 290 million residents, as well as 325,000 hospitalizations. The incidence of food-borne diseases is rising in developing countries, as well as in the developed world. 1 The present study title: A Study to assess the knowledge regarding food hygiene practices among the house wives in slum areas of Pune city. The objective of to assess the knowledge regarding food hygiene practices among the house wives. To associate the knowledge findings with selected demographic variables. Material and Methods: In present study, researcher adopted NonExperimental design. The study carried out on 100 samples. A Non-probability purposive Sampling Technique was used. Data analysis was done mainly using descriptive statistics test- Chai square was applied. Result: In the study 14% of house wives had poor knowledge regarding food hygiene practices 71% of house wives had average knowledge. We can improve their knowledge by providing information and training regarding food hygiene practices. Conclusion: In this study we had found the need of awareness about food hygiene practices and its important and impact on community health and nursing education especially the proper hand wash, take precaution from food contamination via unclean hand, surface, type of food and articles and utensils which are used for cooking.