Abstract :Background: An amputee is a person with a disability who needs special attention because of the condition of the amputation they are experiencing so that it disrupts their quality of life. Amputee uses prosthesis for daily activities. Quality of life is influenced by many factors, by knowing factors affected to prosthetic users quality of life. Purpose: This study aims to conduct an Analysis of factors affected to prosthetic users quality of life in Indonesia. Methods: This is Quantitative research, using observational analytic. It is a crosssectional design. Transfemoral Prosthetic user in Kuspito Clinic is the population. The sampling technique used is a simple random sampling. There were 110 samples. Structural Equation Modeling with AMOS software is used for analysis. The instrument of this research is the questioner. Result: The Questioners are valid and Reliable. The Model purposed is fit. The SEM result shows that motivation, optimism, religiosity, self-efficacy, social support have direct and indirect effect in quality of life. Subjective well-being is the mediator of factors affecting quality of life of prosthetic users. Conclusion: The study showed that motivation, optimism, religiosity, self-efficacy, social support, are the factors affected to prosthetic users quality of life. It is mediated by subjective well-being.