Marine macroalgae or seaweed associated bacteria gains much importance because of its crucial role in the production of certain compounds that are found to be active against various pathogenic bacteria and cancer cells. In this study the antagonistic, antibacterial and anticancer potential of marine macroalgae associated bacteria were analysed. 14 epiphytic and 6 endophytic bacterial strains were isolated. Among the isolated bacteria 3 strains showed antagonistic activity against Staphylococcus aureus and 1 strain showed activity against Escherichia coli. Antibacterial potential of seaweed associated bacteria revealed that the bacterial extracts have the potential to inhibit the growth of Escherichia coli, Bacillus subtilis and Staphylococcus aureus. Further the strains also inhibited the proliferation of SK-MEL-28 cell line in a dose dependent manner.