The purpose of this study was to examine the evolution of antimicrobial activity of flower extract of Hibiscus sabdariffal against antibiotic-resistant Escherichia coli and Staphylococcus aureus. The extract of Hibiscus sabdariffal obtained by rotary and the minimum inhibitory concentrations were investigated to characterize the antimicrobial activities of this extract. Furthermore In the present study, only the susceptibilities of clinical bacterial isolates to the extracts of Hibiscus sabdariffal leaves and flowers have been examined. Therefore, further microbiological studies would be carried out to determine the minimal inhibitory concentrations (MICs) of the extracts prepared from Hibiscus sabdariffal against Escherichia coli and Staphylococcus aureus. The watery extracts of Hibiscus sabdariffal was evaluated against isolates and the results showed that the crude extracts of them with boiling water were more effective than the cold watery extracts.