Background: To assess prevalence of P. aeruginosa and antibiotic sensitivity from respiratory samples. Materials and Methods: One hundred twenty respiratory samples of both genders were taken for study. All samples were processed and Pseudomonas aeruginosa was identified by various biochemical test. Antimicrobial susceptibility testing was done by disk diffusion method as per CLSI. Results: Out of 120 patients, males were 70 (58.3%) and females were 50 (41.7%). Out of 120 respiratory samples, 30 (25%) had P. aeruginosa. Antibiotic sensitivity pattern of Pseudomonas isolates was seen 74% to Ciprofloxacin, 86% to Ofloxacin, 57% to Ceftriaxone, 65% to Ceftazidime, 84% to Amikacin, 85% to Meropenem and 63% to Imipenem. The difference was significant (P< 0.05). Conclusion: There is high prevalence of Pseudomonas aeruginosa in respiratory samples. Increasing resistance of Pseudomonas aeruginosa needs constant surveillance of antimicrobial resistance trends and administration of appropriate antibiotics