Background: Menstruation is a physiological phenomenon which has multiple biophysical and psychosocial elements that have reverberations for a woman of any background. Dysmenorrhea is described as the presence of painful cramps that are of uterine origin occurring during menstruation, representing one of the most common causes of pelvic pain and menstrual disorder. Objectives: This study is intended to assess the association between levels of perceived stress with its effect on the various symptoms of menstrual cycle in medical and non-medical students. Materials and Methods: This is a cross-sectional which was conducted among female students of MBBS, BSc Nursing, Physiotherapy, Art and Science courses of a tertiary care hospital in India. 291 students from the mentioned courses, who are aged above 18 years, after taking their consent were included in the study. Results: 27.15% students are medical students and 31.62% are nursing students, 23.02% of the students are physiotherapy students and 18.21% are art & science students. 53.26% are 1st and 2nd year students. The mean weight of the students was 54.92 ± 10.28 kg and the mean height was 157.18 ± 8.14 cm, whereas mean BMI was 22.28 ± 4.12 Discussion: Our study pointed that mild to moderate severity of pain due to dysmenorrhea reduced the routine physical activity. Risk of dysmenorrhea was twice higher with people of moderate levels of stress in comparison to lower levels, which was in accordance with the study by Wang et al. Conclusion: Early identification and deceleration of the causal factors may have a better preventive role. Programs for stress reduction aiming at reproductive age women, especially for those with a history of dysmenorrhea, can be considered as a possible preventive strategy to reduce the occurrence of dysmenorrhea and associated concerns.