ABSTRACT Early Tooth loss in children caused by caries and dental trauma has a serious impact on their Oral health related quality of life .From the past to till date various approaches such as removable dentures , fixed prosthesis have been into practice to restore the lost tooth structure. Tooth auto transplantation, allotransplantation and dental implants have existed for many years, but not completely successful as they present with problems with biocompatibility , inflammatory changes and failures thus making the problem further more complex . Hence, the development of new methods of tooth replacement has become desirable and is a realistic possibility in children of growing age .Stem cell-based tissue engineering which can recapitulate the in vivo environment showed that dental, non-dental, embryonic and adult stem cells can contribute to teeth formation under favourable circumstances.. The concept of ‘Bioroot’ with supporting Evidence that stem cell populations may be present in human teeth provides the opportunity to consider biological tooth replacement ‘new for old’. This article enlightens on the various materials used in tooth regeneration, their literature evidences and the significance of bioroot formation as potent tooth replacement tool in the future.