The fungus Cryptococcus neoformans is the causative agent of cryptococcosis, which is a systemic opportunistic fungal infection. Cryptococcus neoformans is a capsulated opportunistic yeast fungus that mostly infects HIV-infected and other immunocompromised hosts, causing meningitis or meningoencephalitis in the central nervous system (CNS). A significant contributor to death in immunocompromised patients is the presence of cryptococcal disease in the central nervous system. A prospective study was carried out on a total of 160 patients who were suffering from cryptococcal meningitis and other co-morbid conditions, such as diabetes, the use of corticosteroids, chronic debilitating illness, and HIV/AIDS patients. The patients all had symptoms related to the central nervous system (CNS). The purpose of the study was to identify and isolate Cryptococcus neoformans from cerebrospinal fluid (CSF) samples taken from immunocompromised patients at the Government General Hospital in Guntur who were suspected of having cryptococcal meningitis