Introduction: Pregnancy influences the physical and hormonal status of the body and many women experience it as stressful situaton at some point. Objectives: The present study was aimed to assess and compare the level of stress among primi mother of rural and urban areas during antenatal period. Methods: A descrptive survey was conducted to find out the level of stress. Through appropriate sample size calculation 100 mothers were selected conviniently those were attending antenatal OPD. The questionnaire was developed for measurement of stress based on Wang-34 stress scale. Result: Among the women 84% were form rural and 58% from urban area.In both the groups no one had severe stress. They had moderate level of stress. The mean stress score in rural women was higher than urban women.The difference in level of stress among them was significant at p value < 0.0001. Age, duration of marital life and complication during pregnancy had association with the level of stress. Conclusion: Stress during pregnancy can be dangerous for mother and baby. Finding the ways to reduce the stressful situation is very important for the health of the unborn baby. Counselling services are helpful for managing stress during their pregnancy.