Acorus calamus is familiar for therapeutic plant in the traditional healthcare system of India as well as other countries. The current study is focused in the progress of the phyto-chemical properties and investigation of the therapeutically active compounds that are exist within the methanolic extracts that are attained from the rhizomes of the plant Acorus calamus linn. The Preliminary phyto-chemical screening of the samples in this study unveiled the existence of Terpenoids, Flavanoids, Tannins, Steroids, Saponins and Phenol-based substances. The Thin Layer Chromatography (TLC) is a chromatographic method which is widely utilized in the field of flora-based study. It is a modest, rapid and cost-effective method with the capacity to indicate the constituents present in a crude plant extract. The TLC is widely applicable due to several advantage such as the low cost, quicker analysis, multiple detection capacity and specificized derivation within the similar plate. Amongst the diverse extracts obtained, the methanol extract was utilized for TLC and a novel solvent system was established for the best extraction of the phyto-constituents exist in the extract which was composed of the constituents in the proportion of N-Hexane: Ethyl Acetate: Methanol: Formic acid 3: 4: 3: 0.1 ml for methanol extract. This caused in the documentation of four spots that were present in the Methanol extract. The Rf values of the samples were 0.68, 0.71, 0.79 and 0.81. The research will further deliver information for the precise exploration of the crude floral extract of Acorus calamus linn.