ABSTRACT HBV infection is one of the major concerns and a constant threat to public health. Cytokines are used in biological studies as vital signs in the diagnosis, treatment, and followup of progression a number of diseases such as CHB and HCC . This study aimed to evaluation the levels of HGF , TNF- α , CXCL-13 in HBV patients .Studies have shown that the hepatocyte growth factor is the engine for the development of HCC caused by infection with HBV, and may serve as an effective biomarker for targeted therapy. The study included 100 patients with positive HBs Ag (100% positive). Three cytokines (HGF , TNF- α , CXCL-13) were analyzed in the serum of patients ,and the results showed highly significant increase inthe levels of those cytokines in patients comparing to healthy individuals(p≤0.01). Also ,the results showed there is a highly significant difference(P<0.001) of HGF levels when comparing between acute andchronicHBV patients .Our results concluded HGF , TNF- α , CXCL-13 play a key role in development of HBV.