Aim and objective: To use Diffusion-weighted MRI to evaluate and differentiate spaceoccupying brain lesions in individuals whose conventional MRI (cMRI) assessment revealed aberrant imaging characteristics suggestive of space-occupying brain lesions. Material and Method: We conducted prospective, observational study which enrolled 100 patients had different space occupying lesion detected with DWI. The study was performed on a whole body system at 1.5 Tesla MRI, using a dedicated head coil. Multiplanar T1- and T2-weighted, diffusion, gradient images, using spinecho sequences, post contrast study, and proton magnetic resonance spectroscopy were performed in all cases on single and multivoxels chemical shift imaging. All cases were correlated with histopathology and by follow-up studies after management. Results: Study was done for a period of 1-year in patients of varying clinical symptoms subjected for MRI. Male subjects were more than females. 65% had intra axial whereas 35% had extra axial lesion among space occupying lesion. Abscess highly observed in intra axial whereas meningioma highly observed in extra axial lesions. In our study, tumors, infection, cystic lesions and demyelination type of etiology observed among patients. Tumors were most common overall etiology in our study. Conclusion: Diffusion-weighted magnetic resonance is a useful imaging technique that helps in preoperative differential diagnosis of various space occupying lesions which helps in precise diagnosis and improves the prognosis of many patients with space-occupying lesions.