Introduction: Leprosy is considered a public health issue in countries where the annual prevalence rate is greater than 1 case per 10,000 inhabitants. It is endemic in several countries with low levels of social and economic development, especially India, with the highest absolute number of cases in the former. In 2018, 208,619 new leprosy cases were registered worldwide by the World Health Organization (WHO). Aims & Objectives: to assess bacteriological index in these sample, to correlate these findings with the newer techniques(ELIZA)m