Background: The The purpose of this study was to identify the effects of the education of using structured multimedia on self-efficacy, stress, and performance confidence in future nursing professionals. Methods: A quasi-experimental study with a non-equivalent control group pretest-posttest design was used. Participant were recruited from two nursing colleges. The sample consisted of an intervention group (n=29) that participated in the educational program and a control group (n=30). The data were collected using selfadministered questionnaires. Data analyses utilized x2 -test, fisher's exact probability & ttest with the SPSS 21.0 program. Results: As a result of research, the experimental group showed significantly increases in self-efficacy (t=2.694, p=.027) and performance confidence (t=1.825, p=.031) than the control group. Also, the experimental group showed significantly decreases in stress (t=-4.026, p=.031) than the control group. The findings indicate that the education of using structured multimedia on self-efficacy, stress, and performance confidence in future nursing professionals is an effective teaching method for nursing students. Conclusions: It is important to manage the psychological well-being to prepare successful professionals. In addition, it will be necessary to consider teachinglearning method strategies for applying structured multimedia learning in the nursing education.