Introduction: Orthopedic wards are hazardous places for the acquirement of pathogens, not only pinhole wounds will be infected with microorganisms but inadequately sterilized instruments, environment medical surfaces, and utilization of orthopedic nurses’ knowledge and practice for dressing around pin external fixation without handwashing before any procedure. All of them assist in transmission infection complications from patients to another patient1. Pin site infection is the most common complication. It is documented to be as high as 85% in the Western countries and 71.4% (including major and minor infection) in India2. Objective: The main objective of this study is to assess the knowledge of nursing students before and after the intervention regarding the prevention of pin site infection with external fixators among final year nursing students. Method: A one-group quasiexperimental study design was used for this study. It includes randomization, manipulation, and no control group. The samples for this study were 60 N.D.R.K final nursing students selected through the lottery method. The data collected by using self -structured Knowledge questionnaire on the prevention of pin site infection with external fixators, before introducing the information booklet and three weeks later the post-test was given to the same group. Results: The finding of the pre-test knowledge showed that adequate knowledge in anatomy and physiology of musculoskeletal system (57.3%), moderate knowledge in External fixator (42.0%), and prevention of pin site infection (42.3%).The study related to the level of knowledge regarding prevention of pin site infection among final year b.sc nursing students showed that 61.7% have inadequate knowledge, 38.3% are having moderately adequate knowledge and none are having adequate knowledge. After administering the information booklet 83.3% had adequate knowledge and no one had inadequate knowledge. Considering overall knowledge on pre and post-test scores analyzed by using paired t-test, it showed that the information booklet was effective (0.001) with a mean difference of 12.65 among final year B.sc nursing students.