Introduction: Non-stress’s a method used to test fetal wellbeing before the onset of labor. A prenatal non-stress test functions in overall antepartum surveillance with ultrasound as a part or component of the biophysical profile. Aim: The main aim of this study was to assess knowledge regarding Non stress test and to find association between among staff Nurses. Material/Method: It was a pre experimental study conducted amongst 60 consented nurses of recognized hospital in gurugram. Data was collected through a structured questionnaire and provide self-instructional module on knowledge regarding NST.Result: in that 40% had good knowledge score in pre-test which was improved in post-test that is 90%. Knowledge score. The mean of pre-test mean score was 19.33 which was increased in post-test mean score 24.22 . ʽt’ value for knowledge 11.13 at the level of significance p<0.05. .No association was found between knowledge and other demographic variables. Conclusion: Self Instructional Module will be effective in improving knowledge regarding Non Stress Test (NST) among Staff Nurses in recognized Hospitals at Gurugram.