Online ISSN: 2515-8260

Efficacy of Bougainvillea Spectabilis (Bougainvillea) Bracts Crude Extract As an Alternative to Wright Stain in Blood Smear Preparation

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Hannah Joyce R. Calilung1 , Aileen L. Castillo1 ,Cyra Alexandria U. Gasmido1 , Chelsey Anne S. Legaspi1 and Romy M. Castor1


ABSTRACT In this work, the staining efficacy of Bougainvillea crude extract was evaluated by comparing with the Wright stain as alternative in blood smear preparation. This was studied on the blood collected from 15 non-anemic participants of Lyceum of the Philippines University Cavite. Hematocrit determination was made to confirm if the participants are surely non anemic. Staining pattern of the prepared smear were limited to the effects of Bougainvillea crude extract and Bougainvillea crude extract combine with methylene blue as compared to the standard staining technique, the Wright stain. In addition, the efficacy of Bougainvillea crude extract was further graded by two external and two internal registered medical technologies. Results showed that the staining efficacy of Bougainvillea crude extract is not comparable to the result of Wright stain as all the p-values are less than 0.05. Statistically, the staining capacity of Bougainvillea as alternative to Wright stain showed a significant difference implying that this is not effective in staining the blood smear for morphology evaluation of the blood cells in manual CBC.

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