Abtract: Background: Strength training is recommended for improving muscular strength and slowing deterioration of age-dependent muscle power and for rehabilitating patients with musculoskeletal conditions. Reliable method of assessing muscle strength is essential for standard procedure of strength training protocol for safeguard, sport and rehabilitation. One repetition maximum (1RM) is a reliable means for the measurement of muscle strength and is termed as the value of load against which prescribed movement is achieved with complete full range of motion only once with free weights Objectives: To establish the role of strength training protocol in strengthening muscle assessed by 1 repetition maximum before and after training in adults. Methods: The sample consists of thirty healthy adults were selected randomly on basis of inclusion criteria with age group of 20 to 40 years. The strength training program lasted for 3 weeks (3 × weeks, 60 to 90% of 1RM for 6 to 12 minute, 2 sets, and 10 repetitions) for 5 days a week.1 repetition maximum test (RM) was baseline outcome measure which was assessed for shoulder and elbow flexors and hip and knee extensors before and after strength training. Results: Statistical analysis of data was done by using Paired t-tests to analyze 1RM Pre and post strength training protocol. The results of the study showed a significant improvement in strength in both upper and lower limbs in a short duration of time and the protocol can be used for rehabilitative purposes. Conclusion: There is significant effect of strength training on 1 repetition maximum. This strength protocol can be used for the rehabilitation of various musculoskeletal conditions for the early rehabilitation