In this study, lignin degradation of lignocellulose waste using biopolymer as derived from agro waste. Initially, biopolymer was extracted by optimizing recovery time and rhamnolipid dose. At 50 min of recovery time and 25 % v/v of rhamnolipid dose, a higher of biopolymer (825 mg/g) was derived. The functional group of biopolymer was predicted and it is exhibited the hydroxyalkanoates group. Then, hydroxyalkanoates mediated lignin degradation of lignocellulose waste was proceeded at optimizing conditions.At 27 min of degradation time and 5 mg of hydroxyalkanoates dose, a higher lignin degradation (52 %) were descried which is higher than non treated lignocellulose waste. Further, the energy valuation of hydroxyalkanoates mediated lignin degradation per kg of lignocellulose waste was studied. During energy valuation, energy expended for centrifugation, drying, stiring, incubation, control the temperature of lignocellulose waste and hydroxyalkanoates extraction were considered as the input energy for lignin degradation steps and is measured as 217 kWh. Energy content of lignocellulose sample after lignin degradation was considered as output and is measured as 310 kWh. Net energy was assessed by the difference of output and input energy and is espied as 93 kWh /kg of waste.