The use bee products is know from thousands of years and its usage in treating various pathological condition has been well documented in various religious texts. Apis Mellifera Bee Venom (BV) have 18 pharmacological active compound and is known to treat many diseases such as arthritis and pain. No proven studies of Apis Mellifera Bee Venom on toxicity and peptic ulcer have been carried out. Hence, this study was undertaken to screen BV for acute toxicity and anti-ulcer activity. The Bee Venom was extracted from the bee in a bee farm in Melaka from the bee species of Apis Mellifera. The acute toxicity studies were carried out on mice. Peptic ulcer was induced by 80% ethanol orally and by pyloric ligation. The Sprague Daley rats were pre-treated with bee venom for 7 days via intra-peritoneal (IP). Two different doses of Apis Mellifera Bee Venom, 0.28mg/kg and 0.56mg/kg were used to screen the anti-ulcer activity. The results were evaluated for statistical significant difference by one-way ANOVA with a post hoc Dunnett test. No mortality was witnessed in the animals throughout the treatment for acute toxicity test. Both the dose which is 0.28mg/kg and 0.56mg/kg showed anti-ulcer properties which was evaluated through ulcer index, determination of total acidity and histopathology studies. This result is probably due to the melitin in the Apis Mellifera Bee Venom which reduces the reactive oxidative stress and protects the gastric mucous membrane