Abstract: The article presents the results of a study of acute and chronic toxicity, irritating and allergenic effects of biocoating for the treatment of penetrating eye injuries. It has been established that a bioactive, biodegradable pellicle does not have a toxic effect, is safe and, according to the classification of toxicity, belongs to the 5th group of substances “practically non-toxic substance” and can be recommended for use in the manufacture of medicines intended for the treatment of eye injuries.