ABSTRACT Nowadays, the incidence of Diabetes Mellitus (DM) is growing around the world. In fact, it is one of the most serious diseases among adults in the age of 45 and a life - threatening disease over the age of 50, resulting to be the leading causes of death in which Filipinos are also affected by the disease. One of the most known way to treat DM is using insulin. However, side effect, toxicity, and organ damage may happen due to continuous usage of synthetic antihyperglycemic agent. Therefore, finding non-toxic and affordable treatment for Diabetes such as medicinal plants is presented. Present investigation was undertaken to investigate the ability of Passiflora edulis sims (yellow passion fruit) pulp juice as anti-hyperglycemic agent on male Sprague-dawley rats, and to compare and evaluate which concentration used in the study of Passiflora edulis sims pulp juice is significantly better as anti-hyperglycemic agent. Thirty (30) individual rats were used in this experiment, 10 of them are used for toxicity testing and no toxic effect has been observed after 24 hours. The remaining 20 rats were randomly divided into five (5) groups, DM was induced by using Alloxan monohydrate dissolved in 0.5 M buffered citrate at a pH of 4.5 and was injected intraperitoneally. Treatment was done by oral administration of yellow passion fruit pulp juice, 1ml, 1.5ml, 2ml, and 2.5ml respectively. The effectiveness of fruit juice was compared with a control group this include diabetic rats treated with water. Results of the study revealed that 1.5 mL, 2mL and 2.5mL concentration of the fruit juice significantly decrease the blood glucose of the diabetic rats while pre and post blood glucose only shows a significant decrease on group 4 and group 5, the highly evidence of anti-hyperglycemic factor occur. Therefore, the Passiflora edulis sims pulp juice fruit juice can be used as alternative treatment for diabetes mellitus