Background: The study aimed to compare the awareness levels of the JN1 variant among dental professionals and patients, analyzing perceptions, practices, and implications for infection control within dental healthcare settings. Methods: A comparative cross-sectional study was conducted over six months, involving 300 dental professionals and 500 patients. Surveys and interviews assessed awareness, perceived risk, infection control practices, and demographic information. Quantitative data were analyzed using SPSS, while qualitative data underwent thematic analysis. Results: Dental professionals exhibited significantly higher awareness (85%) of the JN1 variant compared to patients (62%). Additionally, dental professionals demonstrated better understanding, higher perceived risk, and greater adherence to infection control practices than patients. Conclusion: Bridging the awareness gap between dental professionals and patients regarding emerging variants like JN1 is crucial for optimizing infection control measures and promoting a more informed patient population within dental healthcare.