The main objective of the study, the inhibitor effect of Scoparia dulcis L on the crystallization of calcium oxalate in synthetic urine. Our study of the calcium oxalate crystallization is based on the changes in turbidity.The effect of Scoparia dulcis aqueous extract on the crystallization of calcium oxalate was evaluated. The formation of calcium oxalate is caused by the addition of 0.01 M sodium oxalate solution to synthetic urine. The addition of different concentrations of inhibitors (200 μg, 400 μg, 600 μg, 800 μg, 1000 μg, 1200 μg, 1400 μg, 1600 μg, 1800 μg and 2000 μg) allowed us to provide information on the inhibition percentage. The aqueous extract of Scoparia dulcis leaves displayed a maximum nucleation inhibition of 66.928±0.021% observed at 2000 μg concentration. A strong dose-dependent inhibition of the aggregation was shown in the aggregation assay of Scoparia dulcis aqueous extract leaves with a percentage inhibition of 66.51±0.642 percent. In calcium oxalate urinary lithiasis, the aqueous extract of Scoparia dulcis strongly inhibits crystal formation.