Skeletal muscle activity of Capsicum annuum fruit’s extract was studied in the green frog (Rana hexadactyla) by the rectus abdominus muscle preparation. Capsicum annuum fruit’s extract with distilled water 1μg/ml, 10μg/ml and 100μg/ml concentrations. The result indicated that the treatment of Capsicum annuum fruit’s extract alone and combination with acetylcholine produce skeletal muscle activity. Thus from the present study it was concluded that Capsicum annuum fruit’s extract have good skeletal muscle activity alone and in combination with Acetylcholine.