Urinary tract infections (UTIs) are serious health affecting problems worldwide. UTIs cause more than 8.1 million visits to health care providers each year. About 60% of women and 12% of men will have at least one UTI during their lifetime. The extended-spectrum beta-lactamases (ESPL) has limited the use of multiple types of Antibiotics. This research will be carried out in Al-Bashir Hospital. The aim of this study was to identify the prevalence of ESBL in Escherichia coli isolates in the hospital by VITEK. 319 isolate were positive from EBSL from a total of 564. This suggest that a 56% of the patients who has been hospitalized in the time of the study may develop severe urinary tract infections caused by strains of Escherichia coli who have developed resistance to multiple types of antibiotics.