The purpose of analysis work was to develop and optimize drug balls of erythromycin estolate for oral tissue layer delivery of drug for quick delivery of drug and better bio-availability with the aim to treatment of infectious disease. Sodium alginate primarily based drug balls containing Acyclovir were ready by specific methodology. metal alginate, alkyl radical polysaccharide were elite on the premise of formulation of drug balls, mucoadhesive force, In-vitro drug unharness. A thirty two factorial design was utilized for preparation of drug balls whereby the concentration of sodium alginate, concentration of alkyl radical polysaccharide were elite as freelance variables whereas in vitro drug unharness and proportion permeation were the dependent variables. Drug balls were evaluated with relation to the share mucoadhesion, drug content, in-vitro drug unharness and permeation studies. F4 formulation was optimized with mucoadhesive force, permeation potency and In-vitro drug release. Drug balls were characterised by FTIR and DSC study. Stability study revealed that the formulation was found to be stable