Background and aim: Transdermal medication conveyance is one of the sort of organization which has been catching eye over the previous years concerning its potential in ailment treatment. The utilized of entrance enhancers which may improve the bioavailability and broaden the scope of medications are concentrated in the organization of transdermal course. Its utilization in this field might be helpful in reversibly lessen the hindrance capacity of the layer corneum, the furthest layer of the skin. For this analysis, the definition and assessment of transdermal patches of Glibenclamide towards improving saturation through the skin and keeping up the plasma focus utilizing different infiltration enhancers have been researched. Materials and methods: The transdermal were set up by the dissolvable throwing strategy utilizing HPMC and PVA as the polymers notwithstanding the dibutylphthalate as a plasticizer. The infiltration enhancers utilized are oleic corrosive, DMSO, D-limonene, and Sodium Lauryl Sulfate. The transdermal patches have been assessed by their physicochemical properties and in-vitro drug release. Results: The transdermal patches appearance were straightforward and smooth surface, no huge varieties of thickness, weight varieties and collapsing perseverance. Among the definitions considered, F4 which has D-limonene as infiltration enhancers demonstrated great attributes with low dampness misfortune and dampness take-up, most extreme medication arrival of 99.713% and medication content with 92.588% ± 0.8541. Conclusion: The current examinations demonstrated the necessary penetration pace of the medications might be accomplished with the guide of enhancers either physical or substance.