Aim: the aim of the study to assessment of glycated haemoglobin, total protein and albumin levels in patients with type 2 diabetes mellitus. Methods: This case control study was done the Department of Pathology Darbhanga Medical College and Hospital, Laheriasarai, Darbhanga, Bihar, India, for 1 year. The research enlisted 100 participants, 50 of whom were diabetic and 50 of whom were not, all of whom were between the ages of 40 and 70. Every patient's blood sample was obtained in 5mls, with 1ml dispensed into EDTA for glycated haemoglobin estimation and 4ml dispensed into clear containers for serum albumin and total protein estimation. Normal procedures were used to determine the amount of glycated haemoglobin, estimate serum albumin, and estimate total protein. Results: The mean level of HbA1c was significantly higher in the diabetic subjects when compared with control group (10.11±1.41Vs 6.18±0.71; p=0.000). There was no significant differences observed between the age, the serum levels of Albumin and Total protein in the test and control subjects (p>0.05). Conclusion: The present study showed significantly higher mean levels of HbA1c in the diabetic patients compared with the control subjects. However, the mean serum of levels of Albumin and total protein did not differ significantly when compared between the diabetic patients and controls.